CELLULOSE ACETATE ELECTROPHORESIS OF MALATE DEHYDROGENASE
نویسندگان
چکیده
منابع مشابه
Rapid cellulose acetate electrophoresis. I. Serum proteins.
A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and y-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has aff...
متن کاملPrediction of immunoglobulins from patterns of cellulose acetate electrophoresis.
The concentrations of serum immunoglobulins (Ig) C, A, and M were correlated with specific features in the $y region of densitometer patterns of serum protein electrophoresis on cellulose acetate membranes; visual analysis of densitometer patterns permitted semiquantitative prediction of values for lgC and lgA. Important immunoglobulin abnormalities, particularly elevations of IgA, were seen in...
متن کاملRapid electrophoresis and quantitation of haemoglobins on cellulose acetate.
A rapid and reproducible electrophoretic method for the separation and quantitation of haemoglobins on cellulose acetate is described. The accuracy of the method and its possible sources of error are discussed. The normal range for haemoglobin A(2) by this method is 1% to 3% of the total haemoglobin concentration. Blood samples from 32 thalassaemic patients showed haemoglobin A(2) values of 3.5...
متن کاملEstimation of serum proteins by electrophoresis on cellulose acetate.
We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained .albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) X total serum protein concn] and by the formula (absorbance of albumin-bou...
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An immunoelectrophoretic procedure utilizing microprecipitation and cellulose acetate electrophoresis was developed for detection of antibody to specific virus. A model system of tobacco mosaic virus and homologous rabbit antiserum is described.
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ژورنال
عنوان ژورنال: THE JOURNAL OF VITAMINOLOGY
سال: 1964
ISSN: 0022-5398
DOI: 10.5925/jnsv1954.10.179